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Karolinska Institutet, Solna

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Hanna Axelsson^1,2, Helena Almqvist^1,2, Brinton Seashore-Ludlow^1,3

¹Chemical Biology Consortium Sweden, Science for Life Laboratory, Karolinska Institutet, Solna, ²Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna, ³Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, Stockholm

Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).