University of Illinois Cancer Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Giovanni Lugli¹, Yachana Kataria¹, Zachary Richards¹, Peter Gann¹, Xiaofeng Zhou², Larisa Nonn¹

¹Department of Pathology, University of Illinois at Chicago, ²Department of Periodontics, University of Illinois at Chicago

The goal of this protocol is to use laser-capture micro-dissection as an effective method to isolate pure populations of cell types from heterogeneous prostate tissues for downstream RNA analysis.

Bioengineering

Handling and Assessment of Human Primary Prostate Organoid Culture

Tara McCray^*1, Zachary Richards^*1, Joseph Marsili¹, Gail S. Prins^1,2,3, Larisa Nonn^1,3

¹Department of Pathology, University of Illinois at Chicago, ²Departments of Urology, Physiology, and Biophysics, University of Illinois at Chicago, ³University of Illinois Cancer Center

Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.