Tohoku University Graduate School of Dentistry

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Aseel Marahleh¹, Hideki Kitaura¹, Masahiko Ishida¹, Kazuhiro Shima¹, Akiko Kishikawa¹, Saika Ogawa¹, Wei-Ren Shen¹, Jiawei Qi¹, Fumitoshi Ohori¹, Takahiro Noguchi¹, Yasuhiko Nara¹, Itaru Mizoguchi¹

¹Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

This protocol includes dissection of murine long bones and bone marrow isolation, to generate bone marrow macrophages and produce osteoclasts using macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor Kappa-B ligand (RANKL) or tumor necrosis factor alpha (TNF-α) and their arrest by anti-c-fms antibody, M-CSF receptor.

Biology

Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes

Aseel Marahleh¹, Hideki Kitaura¹, Saika Ogawa¹, Wei-Ren Shen¹, Jiawei Qi¹, Fumitoshi Ohori¹, Takahiro Noguchi¹, Yasuhiko Nara¹, Adya Pramusita¹, Ria Kinjo¹, Itaru Mizoguchi¹

¹Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

This protocol describes the dissection of neonatal dmp1-topaz mouse calvaria and isolation of osteocytes expressing the green fluorescent protein through cell digestion and fractionation, in addition to osteocyte preparation for fluorescence activated cell sorting (FACS).