Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

5.9K Views

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07:51 min

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March 18th, 2019

DOI :

10.3791/59089-v

March 18th, 2019

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Aseel Marahleh¹, Hideki Kitaura¹, Masahiko Ishida¹, Kazuhiro Shima¹, Akiko Kishikawa¹, Saika Ogawa¹, Wei-Ren Shen¹, Jiawei Qi¹, Fumitoshi Ohori¹, Takahiro Noguchi¹, Yasuhiko Nara¹, Itaru Mizoguchi¹

¹Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

Summary

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Anti c fms Antibody

Osteoclast Formation

Osteoclast Precursors

Inflammatory Bone Disruption

Bone Marrow Extraction

Experimental Protocol

Dissection

Alpha MEM

Mouse Model

Chapters in this video

0:04

Title

0:56

Murine Hindlimb Dissection and Handling

2:36

Murine Hindlimb Bone Marrow Isolation

3:35

Generation of Bone Marrow Macrophages (BMMs)

5:17

Generation of Osteoclasts from BMMs as Osteoclast Precursors

6:09

Results: Effect of Anti-c-fms Antibody on Osteoclasts

7:35

Conclusion

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