University of Helsinki and Helsinki University Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy

Erika Gucciardo¹, Sirpa Loukovaara², Ani Korhonen¹, Kaisa Lehti^1,3

¹Research Programs Unit, Genome-Scale Biology, Biomedicum Helsinki, University of Helsinki, ²Unit of Vitreoretinal Surgery, Ophthalmology, University of Helsinki and Helsinki University Hospital, ³Department of Microbiology, Tumor, and Cell Biology (MTC), Karolinska Institutet

Here, we present a protocol to study the pathophysiology of proliferative diabetic retinopathy by using patient-derived, surgically-excised, fibrovascular tissues for three-dimensional native tissue characterization and ex vivo culture. This ex vivo culture model is also amenable for testing or developing new treatments.

Medicine

Study Design for Navigated Repetitive Transcranial Magnetic Stimulation for Speech Cortical Mapping

Pantelis Lioumis^1,2, Salla Autti^1,2, Juha Wilenius^2,3, Selja Vaalto^1,2,3, Henri Lehtinen^3,5, Aki Laakso⁴, Erika Kirveskari³, Jyrki P. Mäkelä², Mia Liljeström^1,2, Hanna Renvall^1,2

¹Department of Neuroscience and Biomedical Engineering, Aalto University School of Science, ²BioMag Laboratory, HUS Diagnostic Center, University of Helsinki, Aalto University, and Helsinki University Hospital, ³Department of Clinical Neurophysiology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, ⁴Department of Neurosurgery, University of Helsinki and Helsinki University Hospital, ⁵Department of Child Neurology, University of Helsinki and Helsinki University Hospital

Navigated repetitive transcranial magnetic stimulation is a highly efficient non-invasive tool for mapping speech-related cortical areas. It helps in designing brain surgery and speeds up the direct cortical stimulation conducted during the surgery. This report describes how to perform speech cortical mapping reliably for preoperative evaluation and research.