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University of Helsinki and Helsinki University Hospital

3 ARTICLES PUBLISHED IN JoVE

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Medicine

An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy
Erika Gucciardo 1, Sirpa Loukovaara 2, Ani Korhonen 1, Kaisa Lehti 1,3
1Research Programs Unit, Genome-Scale Biology, Biomedicum Helsinki, University of Helsinki, 2Unit of Vitreoretinal Surgery, Ophthalmology, University of Helsinki and Helsinki University Hospital, 3Department of Microbiology, Tumor, and Cell Biology (MTC), Karolinska Institutet

Here, we present a protocol to study the pathophysiology of proliferative diabetic retinopathy by using patient-derived, surgically-excised, fibrovascular tissues for three-dimensional native tissue characterization and ex vivo culture. This ex vivo culture model is also amenable for testing or developing new treatments.

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Medicine

Study Design for Navigated Repetitive Transcranial Magnetic Stimulation for Speech Cortical Mapping
Pantelis Lioumis 1,2, Salla Autti 1,2, Juha Wilenius 2,3, Selja Vaalto 1,2,3, Henri Lehtinen 3,5, Aki Laakso 4, Erika Kirveskari 3, Jyrki P. Mäkelä 2, Mia Liljeström 1,2, Hanna Renvall 1,2
1Department of Neuroscience and Biomedical Engineering, Aalto University School of Science, 2BioMag Laboratory, HUS Diagnostic Center, University of Helsinki, Aalto University, and Helsinki University Hospital, 3Department of Clinical Neurophysiology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, 4Department of Neurosurgery, University of Helsinki and Helsinki University Hospital, 5Department of Child Neurology, University of Helsinki and Helsinki University Hospital

Navigated repetitive transcranial magnetic stimulation is a highly efficient non-invasive tool for mapping speech-related cortical areas. It helps in designing brain surgery and speeds up the direct cortical stimulation conducted during the surgery. This report describes how to perform speech cortical mapping reliably for preoperative evaluation and research.

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Developmental Biology

Z-Scores for Assessing Ovarian Reserve in Young Patients Undergoing Fertility Preservation
Jasmin Hassan 1,2, Pauliina Damdimopoulou 1,2, Atte Lahtinen 3,4, Kirsi Jahnukainen 5,6, Katri Knuus 7, Ganesh Acharya 1, Richelle D. Björvang 1,8,9
1Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 2Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, 3Applied Tumor Genomics Research Program, Faculty of Medicine, University of Helsinki, 4Department of Medical and Clinical Genetics, Medicum, Faculty of Medicine, University of Helsinki, 5Children's Hospital, Pediatric Research Centre, University of Helsinki and Helsinki University Hospital, 6NORDFERTIL Research Lab Stockholm, Department of Women's, and Children's Health, Karolinska Institutet, and Karolinska University Hospital, 7Department of Obstetrics and Gynecology, Helsinki University Hospital, University of Helsinki, 8Department of Cell and Molecular Biology, Uppsala University, 9Department of Women's and Children's Health, Uppsala University

The present protocol describes a method for assessing ovarian reserve in patients under 25 years old who require fertility preservation through ovarian tissue cryopreservation. This method involves: (1) histological assessment of ovarian reserve in cortical samples, (2) comparison to a reference dataset, and (3) calculation of Z-scores.

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