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Wilkes Honors College and Florida Atlantic University

2 ARTICLES PUBLISHED IN JoVE

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Behavior

Behavioral Approaches to Studying Innate Stress in Zebrafish
Jacqueline S.R. Chin 1, Lydia T. Albert 2, Cody L. Loomis 1,3, Alex C. Keene 1,3, Erik R. Duboué 1,2,3
1Jupiter Life Science Initiative, Florida Atlantic University, 2Wilkes Honors College and Florida Atlantic University, 3Charles E. Schmidt College of Science, Florida Atlantic University

This manuscript describes a simple method to measure stress behaviorally in adult zebrafish. The approach takes advantage of the innate tendency that zebrafish prefer the bottom half of a tank when in a stressful state. We also describe methods for coupling the assay with pharmacology.

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JoVE Journal

Manipulation of Gene Function in Mexican Cavefish
Bethany A. Stahl 1,2, James B. Jaggard 1,2, Jacqueline S.R. Chin 1,2, Johanna E. Kowalko 2,3, Alex C. Keene 1,2, Erik R. Duboué 2,3
1Department of Biological Sciences, Florida Atlantic University, 2Jupiter Life Science Initiative, Florida Atlantic University, 3Harriet L. Wilkes Honors College, Florida Atlantic University

We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

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