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Manipulation of Gene Function in Mexican Cavefish

DOI :

10.3791/59093-v

April 22nd, 2019

April 22nd, 2019

8,555 Views

1Department of Biological Sciences, Florida Atlantic University, 2Jupiter Life Science Initiative, Florida Atlantic University, 3Harriet L. Wilkes Honors College, Florida Atlantic University

We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

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