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Research Institute at Nationwide Children's Hospital

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model
Yong-Ung Lee 1, Tai Yi 1, Shuhei Tara 1, Avione Y. Lee 1, Narutoshi Hibino 1, Toshiharu Shinoka 2, Christopher K. Breuer 1,3
1Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital, 2Department of Cardiothoracic Surgery, Nationwide Children's Hospital, 3Pediatric Surgery, Nationwide Children's Hospital

To improve our knowledge of cellular and molecular neotissue formation, a murine model of the TEVG was recently developed. The grafts were implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. This model achieves similar results to those achieved in our clinical investigation, but over a far shortened time-course.

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Medicine

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation
Yong-Ung Lee 1, Tai Yi 1, Iyore James 1, Shuhei Tara 1, Alexander J. Stuber 1, Kejal V. Shah 1, Avione Y. Lee 1, Tadahisa Sugiura 1, Narutoshi Hibino 2, Toshiharu Shinoka 1, Christopher K. Breuer 1,3
1Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital, 2Cardiothoracic Surgery, Nationwide Children's Hospital, 3Pediatric Surgery, Nationwide Children's Hospital

In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.

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Bioengineering

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
Matthew G. Wiet *1,2, Sayali Dharmadhikari *1,3, Audrey White 1,2, Susan D. Reynolds 4, Jed Johnson 5, Christopher K. Breuer 3,6, Tendy Chiang 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital

Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.

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Engineering

Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
Yifei Liu 1, Yong-Ung Lee 2, Tai Yi 2, Ken Wu 3, Cedric Bouchet-Marquis 3, Han Chan 3, Christopher K. Breuer 2, David W. McComb 1
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering, Ohio State University, 2Center for Regenerative Medicine, Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

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