Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

7.2K Views

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09:57 min

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April 1st, 2019

DOI :

10.3791/59173-v

April 1st, 2019

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Matthew G. Wiet*¹^,², Sayali Dharmadhikari*¹^,³, Audrey White¹^,², Susan D. Reynolds⁴, Jed Johnson⁵, Christopher K. Breuer³^,⁶, Tendy Chiang¹^,³

¹Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, ²The Ohio State University College of Medicine, ³Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, ⁴Center for Perinatal Research, Nationwide Children's Hospital, ⁵Nanofiber Solutions, Inc., ⁶Department of Pediatric Surgery, Nationwide Children's Hospital

Summary

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Tissue engineered Trachea

Electrospinning Parameters

Microsurgical Techniques

Polyethylene Terephthalate PET

Polyurethane PU

Polymer Mixture

Scaffold Sterilization

Airway Regeneration

Host Factors

Chapters in this video

0:04

Title

1:15

Scaffold Manufacturing

2:50

Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest

4:40

Cell Seeding

5:21

Graft Implantation

8:28

Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses

9:27

Conclusion

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