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Phospholamban Foundation

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Gene Transfer for Ischemic Heart Failure in a Preclinical Model
Kiyotake Ishikawa 1, Dennis Ladage 1, Lisa Tilemann 1, Kenneth Fish 1, Yoshiaki Kawase 1, Roger J. Hajjar 1
1Cardiovascular Research Center, Mount Sinai School of Medicine

A method of gene transfer for the treatment of ischemic heart failure is described using a swine model of myocardial infarction. Our simple and reproducible method enables us to readily evaluate the efficacy of various gene transfers with a very simple and reproducible way.

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Medicine

The Left Pneumonectomy Combined with Monocrotaline or Sugen as a Model of Pulmonary Hypertension in Rats
Michael G. Katz 1, Anthony S. Fargnoli 1, Sarah M. Gubara 1, Malik Bisserier 1, Yassine Sassi 1, Charles R. Bridges 1, Roger J. Hajjar 1, Lahouaria Hadri 1
1Cardiovascular Research Center, Department of Cardiology, Icahn School of Medicine at Mount Sinai

The rodent left pneumonectomy is a valuable technique in pulmonary hypertension research. Here, we present a protocol to describe the rat pneumonectomy procedure and postoperative care to ensure minimal morbidity and mortality.

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Medicine

Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model
Olympia Bikou 1, Roger J. Hajjar 2, Lahouaria Hadri 1, Yassine Sassi 1
1Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, 2Phospholamban Foundation

This protocol describes the induction of pulmonary hypertension (PH) in mice based on the exposure to hypoxia and the injection of a VEGF receptor antagonist. The animals develop PH and right ventricular (RV) hypertrophy 3 weeks after the initiation of the protocol. The functional and morphometrical characterization of the model is also presented.

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