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Phospholamban Foundation

3 ARTICLES PUBLISHED IN JoVE

Medicine

Gene Transfer for Ischemic Heart Failure in a Preclinical Model

Kiyotake Ishikawa¹, Dennis Ladage¹, Lisa Tilemann¹, Kenneth Fish¹, Yoshiaki Kawase¹, Roger J. Hajjar¹

¹Cardiovascular Research Center, Mount Sinai School of Medicine

A method of gene transfer for the treatment of ischemic heart failure is described using a swine model of myocardial infarction. Our simple and reproducible method enables us to readily evaluate the efficacy of various gene transfers with a very simple and reproducible way.

Medicine

The Left Pneumonectomy Combined with Monocrotaline or Sugen as a Model of Pulmonary Hypertension in Rats

Michael G. Katz¹, Anthony S. Fargnoli¹, Sarah M. Gubara¹, Malik Bisserier¹, Yassine Sassi¹, Charles R. Bridges¹, Roger J. Hajjar¹, Lahouaria Hadri¹

¹Cardiovascular Research Center, Department of Cardiology, Icahn School of Medicine at Mount Sinai

The rodent left pneumonectomy is a valuable technique in pulmonary hypertension research. Here, we present a protocol to describe the rat pneumonectomy procedure and postoperative care to ensure minimal morbidity and mortality.

Medicine

Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model

Olympia Bikou¹, Roger J. Hajjar², Lahouaria Hadri¹, Yassine Sassi¹

¹Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, ²Phospholamban Foundation

This protocol describes the induction of pulmonary hypertension (PH) in mice based on the exposure to hypoxia and the injection of a VEGF receptor antagonist. The animals develop PH and right ventricular (RV) hypertrophy 3 weeks after the initiation of the protocol. The functional and morphometrical characterization of the model is also presented.