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The Third Affiliated Hospital of Sun Yat-sen University

6 ARTICLES PUBLISHED IN JoVE

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Chemistry

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM
Juan Yao 1, Bruce W. Arey 1, Li Yang 1, Fei Zhang 1, Rachel Komorek 1, Jaehun Chun 1, Xiao-Ying Yu 1
1Earth & Biological Sciences Directorate, Pacific Northwest National Laboratory

We present a procedure for real-time imaging and elemental composition analysis of boehmite particles in deionized water by in situ liquid Scanning Electron Microscopy.

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Developmental Biology

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats
Shihai Jiang *1, Xiaoyou Yi *3, Yuansen Luo 2, Dongjie Yu 1, Yuangao Liu 1, Fei Zhang 1,4, Lei Zhu 2, Kun Wang 1
1Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 2Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 3Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, 4Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University

Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.

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Neuroscience

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions
Benedetta Assetta *1, Changyong Tang *1,2, Jing Bian *3, Ryan O'Rourke 1, Kevin Connolly 1, Thomas Brickler 3, Sundari Chetty 3,4, Yu-Wen Alvin Huang 1,5,6
1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University

The neuron-glial interactions in neurodegeneration are not well understood due to inadequate tools and methods. Here, we describe optimized protocols to obtain induced neurons, oligodendrocyte precursor cells, and oligodendrocytes from human pluripotent stem cells and provide examples of the values of these methods in understanding cell-type-specific contributions in Alzheimer’s disease.

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Medicine

Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
Yuan Sang *1, Junbin Zhang *1, Chang Liu 1, Kaihua Liu 1, Hui Yao 1, Huiqing Zhao 1, Wenbin Xu 1, Yichun Xu 1, Gang Hou 1
1Department of Orthopedics, The Third Affiliated Hospital of Sun Yat-sen University

Presented here is a protocol for using silver nanoparticles to effectively ameliorate the acute symptoms of type II collagenase-induced osteoarthritis mice, including synovial inflammation, synovial hyperplasia, vascular hyperplasia, etc.

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Medicine

The Third Channel-Assisted Unilateral Biportal Endoscopic Technique for Lumbar Spinal Stenosis Combined with Contralateral Disc Herniation
Lixian Tan 1,2,3, Runmin Tang 1,2,3, Limin Rong 1,2,3, Liangming Zhang 1,2,3
1Department of Spine Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 2Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery, 3Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery

Here, we present the third channel-assisted UBE technique, which allows for the vertical removal of herniated disc fragments. This technique can effectively address the limitations of traditional UBE techniques. This article will systematically elaborate on this procedure.

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Medicine

The Examination of Peroxidase-Positive Leukocytes in Semen
Xiaoming Li *1, Xiaohua Cai *1, Liuhong Cai 1, Jianping Ou 1
1Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University

This paper presents an economical and efficient protocol for examining peroxidase-positive leukocytes in semen. With the assistance of a computer-assisted semen analysis (CASA) system, the concentration of peroxidase-positive leukocytes in semen can be obtained within a total of 60 min, effectively improving the efficiency of andrology laboratory and andrologists.

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