Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

4.5K Views

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10:53 min

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November 9th, 2020

DOI :

10.3791/61778-v

November 9th, 2020

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Benedetta Assetta*¹, Changyong Tang*¹^,², Jing Bian*³, Ryan O'Rourke¹, Kevin Connolly¹, Thomas Brickler³, Sundari Chetty³^,⁴, Yu-Wen Alvin Huang¹^,⁵^,⁶

¹Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, ²Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, ³Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, ⁴Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, ⁵Department of Neurology, Warren Alpert Medical School of Brown University, ⁶Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University

Summary

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Human Neurons

Oligodendrocytes

Pluripotent Stem Cells

Neurodegeneration

Neuron oligodendrocyte Interactions

Cellular Differentiation

Extracellular Matrix Solution

Cell Detachment Solution

Cell Culture Protocol

Chapters in this video

0:05

Introduction

0:37

Human Neuron Induction from Human Pluripotent Stem Cells

3:59

Human Oligodendrocyte Precursor Cell (OPCs) Induction from Pluripotent Stem Cells and Oligodendrocyte Maturation

7:05

Co-culturing of Human Induced Neurons (Ins) and Oligodendrocyte Precursor Cells (Iopcs)

8:06

Results: Direct Generation of Human Induced Neurons from Human Pluripotent Stem Cells

10:23

Conclusion

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