Shanghai Institute of Pollution Control and Ecological Security

5 ARTICLES PUBLISHED IN JoVE

Biology

Electron Cryotomography of Bacterial Cells

Songye Chen¹, Alasdair McDowall^1,2, Megan J. Dobro¹, Ariane Briegel^1,2, Mark Ladinsky^1,2, Jian Shi², Elitza I. Tocheva¹, Morgan Beeby^1,2, Martin Pilhofer^1,2, H. Jane Ding¹, Zhuo Li^1,2, Lu Gan¹, Dylan M. Morris¹, Grant J. Jensen^1,2

¹Division of Biology, California Institute of Technology - Caltech, ²Howard Hughes Medical Institute, California Institute of Technology - Caltech

We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.

Environment

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

Zhuo Li^1,2, Fangting Ai³, Jing Zhang⁴, Zhenyang Yu^1,2, Daqiang Yin^1,2

¹College of Environmental Sciences and Engineering, Tongji University, ²Shanghai Institute of Pollution Control and Ecological Security, ³Jiaxing Tongji Institute of Environment, ⁴College of Ecological Technique and Engineering, Shanghai Institute of Technology

Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.

Cancer Research

A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

Zhuo Li^*1, Xiao Yang^*1, Yilu Zhang¹, Xiaolong Yang¹, Xinxin Cui¹, Yanjuan Zhang¹, Wenfeng Gong¹, Huichen Bai¹, Ning Liu¹, Zhiyu Tang¹, Mingming Guo¹, Kang Li¹, Tong Zhang¹, Lai Wang¹, Xiaomin Song¹

¹BeiGene (Beijing) Co., Ltd.

We describe a human peripheral blood mononuclear cell (PBMC) — based humanized xenograft mouse model for translational immuno-oncology research. This protocol could serve as a general guideline for establishing and characterizing similar models for I-O therapy assessment.

Environment

A Dual-Functional Electroactive Filter Towards Simultaneously Sb(III) Oxidation and Sequestration

Fuqiang Liu¹, Fang Li^1,2, Chensi Shen^1,2, Zhiwei Wang^2,3, Wolfgang Sand^1,4, Yanbiao Liu^1,2

¹Textile Pollution Controlling Engineering Center of Ministry of Environmental Protection, College of Environmental Science and Engineering, Donghua University, ²Shanghai Institute of Pollution Control and Ecological Security, ³State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, ⁴Institute of Biosciences, Freiberg University of Mining and Technology

A protocol for the rational design of a dual-functional electroactive filter consisting of carbon nanotubes and titanate nanowires is reported and their environmental applications towards Sb(III) oxidation and sequestration is presented.

Environment

Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae

Bin Zhang^1,2, Xinyue Yang², Jing Zhao³, Ting Xu^2,4, Daqiang Yin^2,4

¹State Key Laboratory of Marine Geology, Tongji University, ²Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, ³Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Center of 10 Shanghai Polytechnic University, ⁴Shanghai Institute of Pollution Control and Ecological Security

A detailed experimental protocol is presented in this paper for the evaluation of neurobehavioral toxicity of environmental pollutants using a zebrafish larvae model, including the exposure process and tests for neurobehavioral indicators.