Electron Cryotomography of Bacterial Cells

25.2K Views

14:23 min

May 6th, 2010

DOI :

10.3791/1943-v

May 6th, 2010


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Electron Cryotomography

Chapters in this video

0:00

Title

0:39

Introduction

2:11

Plunge Freezing Thin Bacterial Cells

3:41

High Pressure Freezing and Cryo Sectioning of Thicker Cells

6:43

Examination of the Cells Using the Cryo Light Microscope

7:49

Collecting Tilt Series Using the Cryo TEM

9:08

Tomogram Reconstruction and Database

10:24

Tomogram Segmentation

10:51

Movies and Animation

11:22

Representative Movies

12:59

Conclusion

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