The Second Xiangya Hospital of Central South University

5 ARTICLES PUBLISHED IN JoVE

Biochemistry

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

Meng Zhao¹, Yawen Tang¹, Patrick J. Ernst^1,2, Asher Kahn-Krell¹, Chengming Fan^1,3, Danielle Pretorius¹, Hanxi Zhu¹, Xi Lou¹, Lufang Zhou², Jianyi Zhang¹, Wuqiang Zhu¹

¹Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, ²Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, ³Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University

In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.

Immunology and Infection

Optimization of the Cuff Technique for Murine Heart Transplantation

Yunhan Ma^*1,2, Baiyi Xie^*1,2, Helong Dai^*3,4,5, Chenxi Wang⁶, Shujiao Liu⁶, Tianshu Lan⁷, Shuangyue Xu^1,2, Guoliang Yan^1,2,6, Zhongquan Qi^1,2,8

¹Organ Transplantation Institute, School of Medicine, Xiamen University, ²Fujian Key Laboratory of Organ and Tissue Regeneration, ³Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, ⁴Clinical Research Center for Organ Transplantation in Hunan Province, ⁵Clinical Immunology Center, Central South University, ⁶School of Medicine, Xiamen University, ⁷Xiamen Medical College, ⁸Medical College, Guangxi University

We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

Medicine

Cox-Maze IV Procedure Concomitant with Valvular Surgery In Situs Inversus Dextrocardia: A Single-Center Experience in China

Cheng Luo^*1, Chengming Fan^*1, Hao Zhang¹, Long Song¹, Yuhong Liu¹, Liming Liu¹

¹Department of Cardiovascular Surgery, the Second Xiangya Hospital, Central South University

We summarize the Cox-Maze IV procedure concomitant with valvular surgery performed in patients with situs inversus dextrocardia at this institution.

Medicine

Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

Xun Wu¹, Kele Qin¹, Di Wang², Kun Xiang¹, Jun Peng², Jianjun Guo³, Jinfu Yang^1,2, Chengming Fan^1,2,3

¹Department of Cardiovascular Surgery, Second Xiangya Hospital, Central South University, ²Hunan Provincial Key Laboratory of Cardiovascular Research, ³Hunan Fangsheng Pharmaceutical Co., Ltd.

Ultrasound-guided cell delivery around the site of myocardial infarction in mice is a safe, effective, and convenient way of cell transplantation.

Medicine

A Surgical Model for Graft of Brown Adipose Tissue on the Heart Surface in Mice

Zhi-Ang Zhou¹, Qin Fu¹, Zi-Han Liu¹, Lei Wang¹, Feng Xu², Xiao Lin³, Ling-Qing Yuan², Zhi-Yuan Zhang¹, Xiao-Bo Liao¹

¹Department of Cardiovascular Surgery, The Second Xiangya Hospital of Central South University, ²National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, ³Department of Radiology, the Second Xiangya Hospital, Central South University

A unique surgical model for grafting brown adipose tissue onto the heart surface in mice was developed. This model may enhance the understanding of the effects of epicardial adipose tissue (EAT) on heart diseases and the cardioprotective role of EAT browning.