Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

6.1K Views

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08:00 min

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July 10th, 2019

DOI :

10.3791/59452-v

July 10th, 2019

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Meng Zhao¹, Yawen Tang¹, Patrick J. Ernst¹^,², Asher Kahn-Krell¹, Chengming Fan¹^,³, Danielle Pretorius¹, Hanxi Zhu¹, Xi Lou¹, Lufang Zhou², Jianyi Zhang¹, Wuqiang Zhu¹

¹Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, ²Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, ³Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University

Summary

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HiPSC derived Cardiomyocytes

Rho Kinase Inhibition

Cell Dissociation Protocol

Purification Medium

RB plus Medium

Verapamil Treatment

Chapters in this video

0:04

Title

1:13

hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation

3:42

Calcium Transient and Contractility Recording

5:21

Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model

7:11

Conclusion

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