National Clinical Research Center for Infectious Diseases

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Weiguang Weng¹, Dongxue Li¹, Cheng Peng¹, Thomas Behnisch¹

¹The Institutes of Brain Science, the State Key Laboratory of Medical Neurobiology, the Collaborative Innovation Center for Brain Science, Fudan University

This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices.

Neuroscience

An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury

Chen Chen^1,2, Yi Ping Zhang³, Yan Sun^1,4, Wenhui Xiong¹, Lisa B. E. Shields³, Christopher B. Shields^3,5, Xiaoming Jin¹, Xiao-Ming Xu¹

¹Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, and Department of Neurological Surgery, Indiana University School of Medicine, ²Program in Medical Neuroscience, Stark Neurosciences Research Institute, Indiana University School of Medicine, ³Norton Neuroscience Institute, Norton Healthcare, ⁴Department of Human Anatomy & Histoembryology, School of Basic Medical Sciences, Fudan University, ⁵Department of Neurological Surgery, University of Louisville School of Medicine

We introduce an in vivo imaging method using two different fluorescent dyes to track dynamic spinal vascular changes following a contusive spinal cord injury in adult Sprague-Dawley rats.

Modified PASM-Masson Staining in a Renal Biopsy

Luo Weiren^*1,2, Yan Sun^*1, Xiaoxing Li^*1, Jizhou Gou^*1, Cheng Peng¹, Hongying Zhang¹, YiKang Sun¹, Xia Zhao¹, Hong Yu¹

¹Department of Pathology, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Diseases, ²Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, School of Medicine of Southern University of Science and Technology

In this study, we present a protocol of PASM-Masson dual staining to identify the location and extent of glomerular immune complex deposition in renal diseases.

Biology

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Jazmine-Saskya N. Joseph-Chowdhury¹, Stephanie Stransky¹, Sarah Graff¹, Ronald Cutler¹, Dejauwne Young¹, Julie S. Kim¹, Carlos Madrid-Aliste¹, Jennifer T. Aguilan¹, Edward Nieves¹, Yan Sun¹, Edwin J. Yoo¹, Simone Sidoli¹

¹Department of Biochemistry, Albert Einstein College of Medicine

This protocol outlines how a three-dimensional cell culture system can be used to model, treat, and analyze chromatin modifications in a near-physiological state.