Biology Centre CAS

2 ARTICLES PUBLISHED IN JoVE

Environment

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

¹Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre CAS

Presented here is a protocol for a single-cell, epifluorescence microscopy-based technique to quantify grazing rates in aquatic predatory eukaryotes with high precision and taxonomic resolution.

Chemistry

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Marcela Nouzova³, Fernando G. Noriega^4,5, Francisco Fernandez-Lima^1,6

¹Deparment of Chemistry and Biochemistry, Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology, Biology Centre CAS, ⁴Department of Biological Sciences, Florida International University, ⁵Department of Parasitology, University of South Bohemia, ⁶Biomolecular Sciences Institute, Florida International University

Presented here is a method for screening lipid structures through stable isotope labeling by using their retention time, mobility, and fragmentation.