Biology Centre CAS
2 ARTICLES PUBLISHED IN JoVE
Environment
Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
Karel Šimek 1, Dagmara Sirova 1
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre CAS
Presented here is a protocol for a single-cell, epifluorescence microscopy-based technique to quantify grazing rates in aquatic predatory eukaryotes with high precision and taxonomic resolution.
Chemistry
Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
Katherine D. Castro 1, Lilian Valadares Tose 1, Matthew Willetts 2, Melvin A. Park 2, Marcela Nouzova 3, Fernando G. Noriega 4,5, Francisco Fernandez-Lima 1,6
1Deparment of Chemistry and Biochemistry, Florida International University, 2Bruker Daltonics Inc., 3Institute of Parasitology, Biology Centre CAS, 4Department of Biological Sciences, Florida International University, 5Department of Parasitology, University of South Bohemia, 6Biomolecular Sciences Institute, Florida International University
Presented here is a method for screening lipid structures through stable isotope labeling by using their retention time, mobility, and fragmentation.
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