Identification of Circular RNAs using RNA SequencingShobana Sekar 1,2, Philipp Geiger 1,2, Lori Cuyugan 1,2, Annalee Boyle 1,2, Geidy Serrano 2,3, Thomas G. Beach 2,3, Winnie S. Liang 1,2
1Translational Genomic Research Institute, 2Arizona Alzheimer's Consortium, 3Banner Sun Health Research Institute
Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.
