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Identification of Circular RNAs using RNA Sequencing Video (Video) | JoVE

DOI :

10.3791/59981-v

November 14th, 2019

November 14th, 2019

11,517 Views

1Translational Genomic Research Institute, 2Arizona Alzheimer's Consortium, 3Banner Sun Health Research Institute

Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.

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