Wallenberg Centre for Molecular Medicine (WCMM)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Alien Balian^*1,2,3, Javier Garcia Gonzalez^*1,2,3, Nora Bastida^1,3, Khadija-Tul Kubra Akhtar^1,3, Baris A. Borsa^1,2,3, Frank J. Hernandez^1,2,3

¹Department of Physics, Chemistry and Biology, Linköping University, ²Wallenberg Centre for Molecular Medicine (WCMM), ³Nucleic Acids Technologies Laboratory (NAT-lab), Linköping University

Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.