XtalPi Inc. (Shenzhen Jingtai Technology Co., Ltd.)

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Isotopic Effect in Double Proton Transfer Process of Porphycene Investigated by Enhanced QM/MM Method

¹State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, ²University of Chinese Academy of Sciences, ³XtalPi Inc. (Shenzhen Jingtai Technology Co., Ltd.), ⁴Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology

A protocol that uses enhanced QM/MM method to investigate the isotopic effect on the double proton transfer process in porphycene is presented here.

Biology

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

Ying Wu^*1, Darwin Diaz^*1, Jian Yin¹, David Bloodgood¹, William Sexton¹, Cheng-I Wei², Shunyuan Xiao^1,3

¹Institute for Bioscience and Biotechnology Research, University of Maryland, ²Department of Nutrition and Food Science, University of Maryland College Park, ³Department of Plant Sciences and Landscape Architecture, University of Maryland College Park

We present a protocol for constructing a simple spore-distribution system consisting of an inoculation box with a ~50 µm mesh and a transparent plastic chamber. This can be used to evenly inoculate plants with powdery mildew spores, thereby enabling accurate and reproducible assessment of disease phenotypes of plants under study.