CSIC-Universidad Autónoma de Madrid

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Ana Bello-Gamboa^*1,2, Juan Manuel Izquierdo^1,2, Marta Velasco^1,2, Solange Moreno^1,2, Alejandro Garrido^1,2, Laura Meyers^1,2, Juan Carlos Palomino³, Víctor Calvo^1,2, Manuel Izquierdo^*1,2

¹Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-Universidad Autónoma de Madrid, ²Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, ³Unidad de Audiovisuales, Facultad de Medicina, Universidad Autónoma de Madrid

This protocol images both the immunological synapse formation and the subsequent polarized secretory traffic towards the immunological synapse. Cellular conjugates were formed between a superantigen-pulsed Raji cell (acting as an antigen-presenting cell) and a Jurkat clone (acting as an effector helper T lymphocyte).