Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in RatsIn Gul Kim 1, Yanru Wu 2, Su A. Park 3, Hana Cho 1, Jung-Woog Shin 2, Eun-Jae Chung 1
1Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University, College of Medicine, 2Department of Biomedical Engineering, Inje University, Gimhae, 3Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials
Esophageal reconstruction is a challenging procedure, and development of a tissue-engineered esophagus that enables regeneration of esophageal mucosa and muscle and that can be implanted as an artificial graft is necessary. Here, we present our protocol to generate an artificial esophagus, including scaffold manufacturing, bioreactor cultivation, and various surgical techniques.
