Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats

7.0K Views

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08:56 min

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February 10th, 2020

DOI :

10.3791/60349-v

February 10th, 2020

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In Gul Kim¹, Yanru Wu², Su A. Park³, Hana Cho¹, Jung-Woog Shin², Eun-Jae Chung¹

¹Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University, College of Medicine, ²Department of Biomedical Engineering, Inje University, Gimhae, ³Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials

Summary

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Tissue engineered Graft

Circumferential Esophageal Reconstruction

Rat Model

Esophageal Repair

Two layered Tubular Scaffold

Nanofiber

3D Printing

Microvascular Anastomosis

Saliva Leakage

Tissue Engineering

Hybrid Scaffold

Bioreactor Cultivation

Adipose derived Stem Cells

Growth Medium

Pulsatile Flow Bioreactor

LIVE DEAD Viability Assay

Surgical Procedure

Chapters in this video

0:04

Introduction

1:27

Cell Seeding and Bioreactor Cultivation

2:51

Surgical Preparation

3:20

Gastrostomy Surgery

4:30

Esophageal Transplantation

6:18

Results: Representative Esophageal Transplantation and Reconstructed Esophagus Histological Analyses

8:02

Conclusion

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