Children's National Hospital

4 ARTICLES PUBLISHED IN JoVE

Medicine

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

Huda Asfour¹, Anastasia M. Wengrowski¹, Rafael Jaimes III¹, Luther M. Swift², Matthew W. Kay¹

¹Electrical and Computer Engineering Department, The George Washington University, ²Pharmacology and Physiology Department, The George Washington University

The objective is to monitor the mitochondrial redox state of isolated hearts within the context of physiologic preload and afterload pressures. A biventricular working rabbit heart model is presented. High spatiotemporal resolution fluorescence imaging of NADH is used to monitor the mitochondrial redox state of epicardial tissue.

Cancer Research

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Raul Vizcardo^*1,2, S.M. Rafiqul Islam^*1,2, Takuya Maeda^1,2, Naritaka Tamaoki^1,2, Meghan L. Good^1,2, Nicholas D. Klemen^1,2, Marta Bosch-Marce^1,2, Li Jia¹, Mikhael J. Kruhlak³, Nicholas P. Restifo^1,2

¹Surgery Branch, National Cancer Institute, NIH, ²Center for Cell-Based Therapy, National Cancer Institute, NIH, ³Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH

This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.

Cancer Research

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Meghan L. Good^*1,2, Raul Vizcardo^*1,2, Takuya Maeda^1,2, Naritaka Tamaoki^1,2, Parisa Malekzadeh¹, Hiroshi Kawamoto³, Nicholas P. Restifo^1,2

¹Surgery Branch, National Cancer Institute, NIH, ²Center for Cell-Based Therapy, National Cancer Institute, NIH, ³Laboratory of Immunology, Institute for Frontier Life and Medical Sciences, Kyoto University

This article describes a method to generate functional tumor antigen-specific induced pluripotent stem cell-derived CD8αβ⁺ single positive T cells using OP9/DLL1 co-culture system.

Medicine

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Luther M. Swift^1,2, Rafael Jaimes III^1,2, Damon McCullough^1,2, Morgan Burke^1,2, Marissa Reilly^1,2, Takuya Maeda^1,2,3, Hanyu Zhang⁴, Nobuyuki Ishibashi^1,2,3, Jack M. Rogers⁴, Nikki Gillum Posnack^1,2,5

¹Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Hospital, ²Children's National Heart Institute, Children's National Hospital, ³Center for Neuroscience Research, Children's National Hospital, ⁴Department of Biomedical Engineering, School of Engineering, University of Alabama at Birmingham, ⁵Department of Pediatrics, Department of Pharmacology & Physiology, School of Medicine and Health Sciences, George Washington University

The objective of this study was to establish a method for investigating cardiac dynamics using a translational animal model. The described experimental approach incorporates dual-emission optocardiography in conjunction with an electrophysiological study to assess electrical activity in an isolated, intact porcine heart model.