Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

12.8K Views

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09:52 min

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November 7th, 2019

DOI :

10.3791/60472-v

November 7th, 2019

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Luther M. Swift¹^,², Rafael Jaimes III¹^,², Damon McCullough¹^,², Morgan Burke¹^,², Marissa Reilly¹^,², Takuya Maeda¹^,²^,³, Hanyu Zhang⁴, Nobuyuki Ishibashi¹^,²^,³, Jack M. Rogers⁴, Nikki Gillum Posnack¹^,²^,⁵

¹Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Hospital, ²Children's National Heart Institute, Children's National Hospital, ³Center for Neuroscience Research, Children's National Hospital, ⁴Department of Biomedical Engineering, School of Engineering, University of Alabama at Birmingham, ⁵Department of Pediatrics, Department of Pharmacology & Physiology, School of Medicine and Health Sciences, George Washington University

Summary

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Optocardiography

Electrophysiology

Langendorff perfused Hearts

Cardiovascular Malfunction

Cardiac Electrophysiology

Modified Krebs Henseleit Medium

Chapters in this video

0:05

Title

1:02

Heart Excision and Langendorff Perfusion

2:42

Electrophysiology Study

5:17

Transmembrane Voltage and Intracellular Calcium Optical Mapping

6:55

Cleanup

7:20

Results: Representative Optical Data from Langendorff-Perfused Model Hearts

8:59

Conclusion

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