Quantification of Tumor Cell Adhesion in Lymph Node CryosectionsElisa Helena Farias Jandrey 1, Mayra Akemi Kuroki 1, Anamaria Aranha Camargo 1, Erico Tosoni Costa 1
1Molecular Oncology Center, Hospital Sírio-Libanês
Here, we describe a simple and inexpensive method that allows the quantification of adhesive tumor cells to lymph node (LN) cryosections. LN-adherent tumor cells are readily identified by light microscopy and confirmed by a fluorescence-based method, giving an adhesion index that reveals the tumor cell-binding affinity to LN parenchyma.
