JoVE Logo
Faculty Resource Center

Sign In

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

DOI :

10.3791/60531-v

February 9th, 2020

February 9th, 2020

10,871 Views

1Molecular Oncology Center, Hospital Sírio-Libanês

Here, we describe a simple and inexpensive method that allows the quantification of adhesive tumor cells to lymph node (LN) cryosections. LN-adherent tumor cells are readily identified by light microscopy and confirmed by a fluorescence-based method, giving an adhesion index that reveals the tumor cell-binding affinity to LN parenchyma.

Tags

Tumor Cell Adhesion

-- Views

Related Videos

article

Lung Tumor Cell Recruitment Assay

article

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

article

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

article

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

article

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

article

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

article

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

article

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

article

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

article

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved