Reims University Hospitals, Robert Debre Hospital

2 ARTICLES PUBLISHED IN JoVE

Genetics

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Aymric Kisserli^1,2, Thierry Tabary^1,2, Jacques Henri Max Cohen^1,2, Valérie Duret^1,2, Rachid Mahmoudi^3,4

¹Department of Immunology, Reims University Hospitals, Robert Debré Hospital, ²Faculty of Medicine, LRN EA 4682, University of Reims Champagne-Ardenne, ³Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, ⁴Faculty of Medicine, EA 3797, University of Reims Champagne-Ardenne

Here, we describe an innovative method to determine complement receptor 1 (CR1) length polymorphisms for use in several applications, particularly the assessment of susceptibility to diseases such as Alzheimer's disease (AD). This method could be useful to better understand the role of CR1 isoforms in the pathogenesis of AD.

Immunology and Infection

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Aymric Kisserli^1,2, Sandra Audonnet³, Valérie Duret^2,4, Thierry Tabary^2,4, Jacques Henri Max Cohen², Rachid Mahmoudi^5,6

¹Oncogeriatric Coordination Unit, Reims University Hospitals, Maison Blanche Hospital, ²Faculty of Medicine, University of Reims Champagne-Ardenne, ³URCACyt, Flow cytometry technical platform, University of Reims Champagne-Ardenne, ⁴Department of Immunology, Reims University Hospitals, Robert Debre Hospital, ⁵Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, ⁶Faculty of Medicine, University of Reims Champagne-Ardenne

The aim of this method is to determine the CR1 density in the erythrocytes of any subject by comparing with three subjects whose erythrocyte CR1 density is known. The method uses flow cytometry after immunostaining of the subjects' erythrocytes by an anti-CR1 monoclonal antibody coupled to an amplified system using phycoerythrin (PE).