This protocol can be used to measure the number of antigenic sites on cellular receptor of interest. The main advantage of this technique is that it produces robust results, even for receptors expressed at a low density. This method enables the ev
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The aim of this method is to determine the CR1 density in the erythrocytes of any subject by comparing with three subjects whose erythrocyte CR1 density is known. The method uses flow cytometry after immunostaining of the subjects' erythrocytes by an anti-CR1 monoclonal antibody coupled to an amplified system using phycoerythrin (PE).