University Hospital of Tübingen

5 ARTICLES PUBLISHED IN JoVE

Medicine

Evaluation of Patients' Posture and Gait Profile After Lumbar Fusion Surgery by Video Rasterstereography and Treadmill Gait Analysis

Sebastian Scheidt^1,2, Ulf Krister Hofmann², Falk Mittag²

¹Department of Orthopedics and Trauma Surgery, University Hospital Bonn, ²Department of Orthopedic Surgery, University Hospital Tuebingen

Here, we present a protocol to analyze the posture and the gait of patients after lumbar fusion surgery by means of high-resolution video rasterstereography and a treadmill equipped with an integrated sensor mat. Allowing critical functional postoperative evaluation on a less subjective level may enhance accuracy and reliability of indication for surgery.

Bioengineering

Application of Atomic Force Microscopy to Detect Early Osteoarthritis

Marina Danalache^*1, Aadhya Tiwari^*1, Viktor Sigwart^1,2, Ulf Krister Hofmann^1,3

¹Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, ²Medical faculty of the University of Tübingen, ³Department of Orthopaedic Surgery, University Hospital of Tübingen

We present a method to investigate early osteoarthritic changes at the cellular level in articular cartilage by using atomic force microscopy (AFM).

Biology

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Florian Christof Bonnaire^1,2, Martina Feierabend³, Julius Michael Wolfgart^1,4, Wolfram Breuer⁵, Christian Walter², Ulf Krister Hofmann^1,2, Marina Danalache¹

¹Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, ²Department of Orthopedic Surgery, University Hospital of Tübingen, ³Institute for Bioinformatics and Medical Informatics, Faculty of science of the University of Tübingen, ⁴Medical faculty of the University of Tübingen, ⁵Bavarian Health and Food Safety Authority

We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.

Bioengineering

Injection of Porcine Adipose Tissue-Derived Stroma Cells via Waterjet Technology

Jasmin Knoll^*1, Marina Danalache^*2, Walter Linzenbold³, Markus Enderle³, Tanja Abruzzese¹, Arnulf Stenzl¹, Wilhelm K. Aicher¹

¹Department of Urology, University Hospital Tübingen, ²Department of Orthopaedic Surgery, University Hospital Tübingen, ³ERBE Elektromedizin GmbH Tübingen

We present a method of cell injection via needle free waterjet technology coupled with a sequela of post-delivery investigations in terms of cellular viability, proliferation, and elasticity measurements.

Cancer Research

5-Aminolevulinic Acid-mediated Photodynamic Therapy on Primary Bone Tumor and Bone Metastases Cell Lines

Saskia Magdalen Sachsenmaier^*1,2, Christian Walter^*2, Chen Liang^1,2, Rosa Riester¹, Nikolaus Wülker^1,2, Marina Danalache¹

¹Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, ²Department of Orthopaedic Surgery, University Hospital of Tübingen

This protocol presents a step-by-step methodological approach of exposing bone metastatic cells lines and primary bone tumors to 5-aminolevulinic acid-mediated photodynamic therapy (PDT). The effects on cell migration potential/invasiveness, viability, apoptosis, and senescence potential are also analyzed following PDT exposure.