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University Hospital of Tübingen

5 ARTICLES PUBLISHED IN JoVE

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Medicine

Evaluation of Patients' Posture and Gait Profile After Lumbar Fusion Surgery by Video Rasterstereography and Treadmill Gait Analysis
Sebastian Scheidt 1,2, Ulf Krister Hofmann 2, Falk Mittag 2
1Department of Orthopedics and Trauma Surgery, University Hospital Bonn, 2Department of Orthopedic Surgery, University Hospital Tuebingen

Here, we present a protocol to analyze the posture and the gait of patients after lumbar fusion surgery by means of high-resolution video rasterstereography and a treadmill equipped with an integrated sensor mat. Allowing critical functional postoperative evaluation on a less subjective level may enhance accuracy and reliability of indication for surgery.

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Bioengineering

Application of Atomic Force Microscopy to Detect Early Osteoarthritis
Marina Danalache *1, Aadhya Tiwari *1, Viktor Sigwart 1,2, Ulf Krister Hofmann 1,3
1Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, 2Medical faculty of the University of Tübingen, 3Department of Orthopaedic Surgery, University Hospital of Tübingen

We present a method to investigate early osteoarthritic changes at the cellular level in articular cartilage by using atomic force microscopy (AFM).

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Biology

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
Florian Christof Bonnaire 1,2, Martina Feierabend 3, Julius Michael Wolfgart 1,4, Wolfram Breuer 5, Christian Walter 2, Ulf Krister Hofmann 1,2, Marina Danalache 1
1Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, 2Department of Orthopedic Surgery, University Hospital of Tübingen, 3Institute for Bioinformatics and Medical Informatics, Faculty of science of the University of Tübingen, 4Medical faculty of the University of Tübingen, 5Bavarian Health and Food Safety Authority

We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.

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Bioengineering

Injection of Porcine Adipose Tissue-Derived Stroma Cells via Waterjet Technology
Jasmin Knoll *1, Marina Danalache *2, Walter Linzenbold 3, Markus Enderle 3, Tanja Abruzzese 1, Arnulf Stenzl 1, Wilhelm K. Aicher 1
1Department of Urology, University Hospital Tübingen, 2Department of Orthopaedic Surgery, University Hospital Tübingen, 3ERBE Elektromedizin GmbH Tübingen

We present a method of cell injection via needle free waterjet technology coupled with a sequela of post-delivery investigations in terms of cellular viability, proliferation, and elasticity measurements.

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Cancer Research

5-Aminolevulinic Acid-mediated Photodynamic Therapy on Primary Bone Tumor and Bone Metastases Cell Lines
Saskia Magdalen Sachsenmaier *1,2, Christian Walter *2, Chen Liang 1,2, Rosa Riester 1, Nikolaus Wülker 1,2, Marina Danalache 1
1Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, 2Department of Orthopaedic Surgery, University Hospital of Tübingen

This protocol presents a step-by-step methodological approach of exposing bone metastatic cells lines and primary bone tumors to 5-aminolevulinic acid-mediated photodynamic therapy (PDT). The effects on cell migration potential/invasiveness, viability, apoptosis, and senescence potential are also analyzed following PDT exposure.

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