In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse AstrocytesLaura Dumas *1, Solène Clavreul *2, Jason Durand 1, Edwin Hernandez-Garzon 3, Lamiae Abdeladim 4, Raphaëlle Barry-Martinet 2, Alicia Caballero-Megido 1, Emmanuel Beaurepaire 4, Gilles Bonvento 3, Jean Livet 2, Karine Loulier 1
1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris
Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline.
