In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

5.4K Views

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06:47 min

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May 21st, 2020

DOI :

10.3791/61110-v

May 21st, 2020

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Laura Dumas*¹, Solène Clavreul*², Jason Durand¹, Edwin Hernandez-Garzon³, Lamiae Abdeladim⁴, Raphaëlle Barry-Martinet², Alicia Caballero-Megido¹, Emmanuel Beaurepaire⁴, Gilles Bonvento³, Jean Livet², Karine Loulier¹

¹Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, ²Sorbonne Université, INSERM, CNRS, Institut de la Vision, ³Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, ⁴Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris

Summary

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In Utero Electroporation

MAGIC Markers

Cortical Astrocytes

Morphology Assessment

Progenitor Cells

Surgical Tools Sterilization

Micropipette Technique

DNA Solution Injection

Pregnant Mouse Preparation

Electroporation Procedure

Embryonic Day 15 5 Embryo

Lateral Ventricle Injection

Chapters in this video

0:04

Introduction

0:43

Surgical Preparation

1:39

Pregnant Mouse Preparation

2:16

Intraventricular Injection

3:16

In Utero Electroporation (IUE)

4:24

Multichannel Confocal Imaging

5:23

Results: Representative Cortical Astrocyte Segmentation and Arborization

6:10

Conclusion

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