TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

Childérick Severac^*1, Sergio Proa-Coronado^*1,2,3, Cécile Formosa-Dague⁴, Adrian Martinez-Rivas^3,5, Etienne Dague²

¹ITAV-CNRS, Université de Toulouse, CNRS, Toulouse, France, ²LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France, ³ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico, ⁴TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France, ⁵CIC, Instituto Politécnico Nacional (IPN), Mexico City, Mexico

This protocol aims to automate AFM measurements on hundreds of microbial cells. First, microbes are immobilized into PDMS stamp microstructures and then force spectroscopy measurements are performed automatically on hundreds of immobilized cells.