Universidad del Valle de México

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Quantitation of Rabies Virus in Various Bovine Brain Structures

Edith Rojas-Anaya¹, Daniela Anaya-Razo², Isabel Bárcenas-Reyes³, Elizabeth Loza-Rubio¹

¹Centro Nacional de Investigación Disciplinaria en Microbiología Animal (CENID-MA), INIFAP, ²Campus Toluca, Universidad del Valle de México, ³Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro

This protocol presents a qRT-PCR-based approach for determining the rabies virus nucleoprotein (N) gene copy number within various bovine brain anatomical structures using in vitro transcription.