Poznań University of Physical Education

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation

Marcin Bączyk^*1, Piotr Krutki^*1

¹Department of Neurobiology, Poznań University of Physical Education

This protocol describes in vivo intracellular recording of rat lumbar motoneurons with simultaneous trans-spinal direct current stimulation. The method enables us to measure membrane properties and to record rhythmic firing of motoneurons before, during and after anodal or cathodal polarization of the spinal cord.

Neuroscience

Functional Isolation of Single Motor Units of Rat Medial Gastrocnemius Muscle

Hanna Drzymała-Celichowska^*1,2, Jan Celichowski^*1

¹Department of Neurobiology, Poznań University of Physical Education, ²Department of Biochemistry, Poznań University of Physical Education

This method allows the recording of the force of twitch and tetanic contractions and action potentials in three types of motor units in the rat medial gastrocnemius muscle. The functional isolation of a single motor unit is induced by electrical stimulation of the axon.