Regeneron Pharmaceuticals, Inc.

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Flow Cytometric Characterization of Murine B Cell Development

Faith M. Harris^*1, Karoline A. Meagher^*1, Maggie Zhong¹, Benjamin J. Daniel², Mark Eckersdorff¹, Jesse A. Green¹, Vera Voronina¹, Chunguang Guo¹, Andre Limnander³, Lynn E. Macdonald¹

¹Regeneron Tech Centers, Regeneron Pharmaceuticals, Inc., ²Regeneron Therapeutic Proteins, Regeneron Pharmaceuticals, Inc., ³Immunology and Inflammation, Regeneron Pharmaceuticals, Inc.

We describe herein a simple analysis of the heterogeneity of the murine immune B cell compartment in the peritoneum, spleen, and bone marrow tissues by flow cytometry. The protocol can be adapted and extended to other mouse tissues.