Flow Cytometric Characterization of Murine B Cell DevelopmentFaith M. Harris *1, Karoline A. Meagher *1, Maggie Zhong 1, Benjamin J. Daniel 2, Mark Eckersdorff 1, Jesse A. Green 1, Vera Voronina 1, Chunguang Guo 1, Andre Limnander 3, Lynn E. Macdonald 1
1Regeneron Tech Centers, Regeneron Pharmaceuticals, Inc., 2Regeneron Therapeutic Proteins, Regeneron Pharmaceuticals, Inc., 3Immunology and Inflammation, Regeneron Pharmaceuticals, Inc.
We describe herein a simple analysis of the heterogeneity of the murine immune B cell compartment in the peritoneum, spleen, and bone marrow tissues by flow cytometry. The protocol can be adapted and extended to other mouse tissues.
