Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional

2 ARTICLES PUBLISHED IN JoVE

Biology

Size Determination and Phenotypic Analysis of Urinary Extracellular Vesicles using Flow Cytometry

Itze Cecilia Navarro-Hernandez^1,2, Ernesto Acevedo-Ochoa¹, Guillermo Juárez-Vega¹, David Eduardo Meza-Sánchez¹, José Manuel Hernández-Hernández², José Luis Maravillas-Montero¹

¹Red de Apoyo a la Investigación, Universidad Nacional Autónoma de México e Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, ²Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional

This protocol describes a method for the isolation of urinary extracellular vesicles, uEVs, from healthy human donors and their phenotypic characterization by the size and surface marker expression using flow cytometry.

Immunology and Infection

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Alexia Almaraz-Arreortua¹, Sorely Adelina Sosa-Luis², William de Jesús Ríos-Ríos¹, María de los Ángeles Romero-Tlalolini³, Sergio Roberto Aguilar-Ruiz⁴, Rafael Baltiérrez-Hoyos³, Honorio Torres Aguilar¹

¹Clinical Immunology Research Department of Biochemical Sciences Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, ²Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, ³CONACYT- Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, ⁴Molecular Immunology Research Department of Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca

Presented here is a protocol for the induction and analysis of in vitro neutrophil extracellular traps (NETs). Quantification of DNA, cathelicidin (LL37), and enzyme activity yielded data that show the variability in the composition and morphology of NETs induced by microbial and chemical stimuli under similar controlled conditions.