Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Mégane Bregeon¹, Nicolas Guyot¹, Sophie Réhault-Godbert¹

¹Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

This article reports a simple method to isolate the outer and inner perivitelline sublayers of chicken eggs while minimizing structural alteration and to optimize protein solubilization of each sublayer for proteomic analyses.