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United Arab Emirates University

3 ARTICLES PUBLISHED IN JoVE

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Biology

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
Amnah Alzahmi 1,2, Sarah Daakour 1, Diana Charles El Assal 3, Bushra S. Dohai 3, Amphun Chaiboonchoe 3, Weiqi Fu 3,4, David R. Nelson 1, Alexandra Mystikou 1, Kourosh Salehi-Ashtiani 1,3
1Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Research Institute, 2Department of Biology, United Arab Emirates University, 3Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math, New York University Abu Dhabi, 4Department of Marine Science, Ocean College, Zhejiang University

This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network model.

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Biology

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
Muhammad Abid Sheikh 1, Suraiya Anjum Ansari 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency.

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Biology

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
Muhammad Abid Sheikh 1, Farah Helena Afandi 1, Grazia Iannello 2, Barbara Corneo 2, Bright Starling Emerald 3,4,5, Suraiya Anjum Ansari 1,4,5
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Columbia Stem Cell Initiative, Stem Cell Core, Columbia University Irving Medical Center, 3Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, 4Zayed Center for Health Sciences, United Arab Emirates University, 5ASPIRE Precision Medicine Research Institute Abu Dhabi (PMRI-AD), United Arab Emirates University

The presented method describes the generation of a CRISPR-mediated gene knockout in the human embryonic stem cell (hESC) line H9, which stably expresses sgRNAs targeting the L2HGDH gene using a highly efficient lentiviral-mediated gene delivery system.

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