Pflanzenwissenschaften (IBG-2), Forschungszentrum Jülich
2 ARTICLES PUBLISHED IN JoVE
Environment
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Megan Garvey 1, Johannes Klinger *1, Holger Klose *1, Rainer Fischer 2, Ulrich Commandeur 1
1Institute for Molecular Biotechnology, Bio7, RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.
Chemistry
Fractionation of Lignocellulosic Biomass using the OrganoCat Process
Leonie Schoofs 1, Dennis Weidener 1, Ulrich Schurr 1, Holger Klose 1, Philipp M. Grande 1
1Institut für Bio- und Geowissenschaften, Pflanzenwissenschaften (IBG-2), Forschungszentrum Jülich
OrganoCat is a method for the pretreatment and fractionation of lignocellulose under mild conditions into lignin, fermentable sugars, and cellulose pulp. In a biogenic, biphasic solvent system of water and 2-methyltetrahydrofuran with 2,5-furancarboxylic acid as catalyst, the OrganoCat products are separated in situ for straightforward product recovery.
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