NUS Graduate School for Integrative Sciences and Engineering

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Polymeric Microneedle Array Fabrication by Photolithography

Himanshu Kathuria¹, Jaspreet Singh Kochhar¹, Michelle Hui Min Fong¹, Michinao Hashimoto², Ciprian Iliescu³, Hanry Yu^3,4,5, Lifeng Kang¹

¹Department of Pharmacy, National University of Singapore, ²Singapore University of Technology and Design, ³Institute of Bioengineering and Nanotechnology, Agency for Science, Technology and Research (A*STAR), ⁴Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, ⁵Mechanobiology Institute, National University of Singapore

Here, we present a protocol describing a mold-free fabrication process of the polymeric microneedles by photolithography.

Biology

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Inn Chuan Ng^*1, Li Zhang^*2, Narelle Nichola Yi Ying Shen³, Yun Ting Soong⁴, Chan Way Ng⁵, Phoebe Kang Sheing Koh¹, Yan Zhou³, Hanry Yu^1,3,4,5,6

¹Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, ²College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, ³Mechanobiology Institute, National University of Singapore, ⁴Institute of Bioengineering and Nanotechnology, A*STAR, ⁵NUS Graduate School for Integrative Sciences and Engineering, ⁶CAMP, Singapore-MIT Alliance for Research and Technology

This protocol details the use of a special intravenous catheter, standardized sterile disposable tubing, temperature control complemented by real-time monitoring, and an alarm system for two-step collagenase perfusion procedure to improve the consistency in the viability, yield, and functionality of isolated primary rat hepatocytes.