National Environmental Agency

1 ARTICLES PUBLISHED IN JoVE

Biology

Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes

Irene Li^*1, Keng Wai Mak^*1, Jeslyn Wong¹, Cheong Huat Tan¹

¹Environmental Health Institute, National Environmental Agency

Here, we present a protocol for studying mating competitiveness of male Aedes aegypti using fluorescent dye as a marker. Female mosquitoes are exposed to both marked and unmarked males for copulation. Post mating, their spermathecae are examined under a fluorescence microscope to determine their mating partner.