National Environmental Agency

1 ARTICLES PUBLISHED IN JoVE

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Biology

Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes
Irene Li *1, Keng Wai Mak *1, Jeslyn Wong 1, Cheong Huat Tan 1
1Environmental Health Institute, National Environmental Agency

Here, we present a protocol for studying mating competitiveness of male Aedes aegypti using fluorescent dye as a marker. Female mosquitoes are exposed to both marked and unmarked males for copulation. Post mating, their spermathecae are examined under a fluorescence microscope to determine their mating partner.

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